几种中药材对兔原代肾小管上皮细胞增殖作用影响的研究

发布时间:2019-08-29 来源: 日记大全 点击:

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  摘要:通过体外培养兔原代肾小管上皮细胞,来研究大血藤、吴茱萸、豨莶草及川牛膝4种中药材提取物对肾小管上皮细胞增殖作用的影响。利用MTT法测定大血藤、吴茱萸、豨莶草及川牛膝4种中药材的水提与醇提的3mg/L,30mg/L和300mg/L三个浓度组对兔原代肾小管上皮细胞增殖作用的影响。结果表明,吴茱萸和豨莶草的醇提300mg/L浓度组及大血藤的水提300mg/L浓度组对兔原代肾小管上皮细胞的增殖作用抑制很强,其存活率分别仅有24.12%,16.03%和30.84%,而大血藤的醇提3mg/L浓度组和豨莶草的醇提30mg/L及3mg/L浓度组对兔原代肾小管上皮细胞的增殖作用具有促进作用,其存活率达到了107.91%,114.95%和111.45%。大血藤、吴茱萸、豨莶草及川牛膝4种中药材对兔原代肾小管上皮细胞增殖作用均有不同程度的影响。
  关键词:大血藤;吴茱萸;豨莶草;川牛膝;增殖作用
  中图分类号:R914
  文献标识码:A
  文章编号:1008-0457(2017)04-0076-04 国际DOI编码:10.15958/j.cnki.sdnyswxb.2017.04.014
  Abstract:The effects of extracts of four Chinese herbal medicines, namely Sargentodoxa cuneata、Tetradium ruticarpum、Siegesbeckia orientalisL and Cyathula officinalis Kuan, on the proliferation of renal tubular epithelial cells were studied by culturing rabbit primary renal tubular epithelial cells in vitro. The effects of 3mg/L, 30 mg / L and 300 mg / L of water extraction and alcohol extraction of the four herbal medicines on tubule epithelial cell proliferation were studied by MTT method. The results showed that 300 mg / L of the alcohol extract of T. ruticarpum and S. orientalis and the water extract of 300 mg / L of S. cuneata significantly inhibited the proliferation of primary renal tubular epithelial cells by 24.04%, 16.03% and 30.84%, respectively. 3 mg / L of the alcohol extract of S. cuneata and 30 mg / L and 3 mg / L of alcohol extract of S. orientalis showed a proliferation effect on primary renal tubular epithelial cells, and the survival rate reached 107.91%, 114.95% and 111.45%. All of the four Chinese herbal medicines had some effects on the proliferation of primary renal tubular epithelial cells in rabbit.
  Key words:sargentodoxa cuneata; tetradium ruticarpum; siegesbeckia orientalisL; cyathula officinalis kuan; proliferation effect
  大血藤(Sargentodoxa cuneata),木通科大血藤属植物,具有抗炎,活血,祛风止痛等药理作用。吴茱萸(Tetradium ruticarpum),芸香科吴茱萸属植物,具有止泻、散寒止痛等药理作用。豨签草(Siegesbeckia orientalis L.)为菊科草本植物,具有祛风湿,利关节,抗炎等药理作用。川牛膝(Cyathula officinalis Kuan),苋科杯苋属植物,具有逐瘀通经,通利关节,降血压作用等药理作用[1]。这4种中药材均具有温补肾阳、利水渗湿、益气活血等功效,根据中医药理论中的性味归经之说,均可归属为肾经。根据归经理论,能够将药物的作用与人体的脏腑经脉之间的关系相结合,说明药物能治疗或作用的脏腑经脉的病变,从而就能具体的认识到药物的主要作用器官和药物的特殊功效[2]。本文旨在利用原代分离的兔肾小管上皮细胞对大血藤、吴茱萸、豨莶草及川牛膝4种中药材提取物对肾小管上皮細胞增殖作用的影响进行研究,为这4种中药材的应用及肾毒性作用的研究提供参考。
  1 材料与方法
  1.1 材料
  20天龄雄兔购自贵州省畜牧研究所实验基地,清洁级,合格证CSXK(黔)2012-001。
  中药材:大血藤、吴茱萸(四平市百草堂中药饮片有限公司);豨莶草(四川省川南药业有限公司);川牛膝(成都市益诚药业有限公司)。
  试剂:DMEM/F12(1∶1)(Hyclone),胎牛血清(四季青),胰蛋白酶(Amresco),碱性磷酸酶染液—钙钴法(南京建成),SP染液、CK18(北京博奥森),DAB显色剂(北京中杉金桥),噻唑蓝(MTT),二甲亚砜(DMSO)。

相关热词搜索:肾小管 增殖 几种 中药材 作用

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