三七皂苷对硫化氢致豚鼠急性肺损伤的治病机制的研究

发布时间:2018-06-23 来源: 感恩亲情 点击:


  [摘要] 目的 观察三七皂苷(SAT)对豚鼠肺损伤的治疗作用并研究其作用机制。 方法 120只豚鼠随机分为T0组、TS组、SAT组、FS组。采用硫化氢制备豚鼠肺损伤模型,构模8 h后停止给药,乙醚麻醉动物,分别于7 h、14 h、21 h及24 h时间点观测其肺损伤程度及病理变化。 结果 在各时间点观察:SAT能有效降低SWW浓度及肺静脉高压,与T0组、TS组比较差异具有统计学意义(P<0.05);但SAT与激素联合治疗效果差异并不明显,与FS组比较差异无统计学意义(P>0.05)。 结论 SAT能有效抑制硫化氢引起的肺损伤进程。
  [关键词] 三七总皂苷;硫化氢;急性肺损伤;肺静脉压
  [中图分类号] R285.5 [文献标识码] A [文章编号] 1673-9701(2018)08-0032-03
  Mechanism of panax notoginseng saponins on acute lung injury caused by hydrogen sulfide in guinea pigs
  TAO Zhenghua LIU Hongquan
  Hun"nan District Central Hospital of Shenyang City, Shenyang 110000, China
  [Abstract] Objective To investigate the effective and mechanism of panax notoginseng saponins(SAT) on acute lung injury(ALI) caused by hydrogen sulfide in guinea pigs. Methods 120 guinea pigs were randomized into group T0, group TS, group SAT and group FS. The ALI model of guinea pigs was established by hydrogen sulfide. The drug was removed 8 hours after the model was established. The animals were anaesthetized by aether. The degree of lung injury and pathological changes were observed at 7th, 14th, 21st and 24th hour. Results Compared with group T0 and TS, SAT could decrease the level of SWW and high pulmonary venous pressure significantly at every time point(P<0.05). However, compared with group FS, the effectiveness of SAT was not significantly different from that of combined treatment of hormone(P>0.05). Conclusion SAT could inhibit the process of lung injury caused by hydrogen sulfide effectively.
  [Key words] Total saponins of panax notoginseng; Hydrogen sulfide; Acute lung injury; Pulmonary venous pressure
  三七(Pseudo-ginseng)作为一种重要中药材,得到临床广泛的应用,对其药理研究也被现在的中外学者不断论述,但是多都停留在分子前水平,通常为三七总皂苷(SAT)[1]。本实验即在此基础上探寻SAT对阻止一些急性损伤因素中所起的关鍵作用[2]。肺水肿常是某些损伤性疾病的前期症状,尽管病因多种,但病理基础基本相同[3],实验中以硫化氢作为致模药物,选择豚鼠构造肺水肿模型[4],实验前经过反复测试得出标准构模时间、标本重量、药物浓度等重要参数,实验证实三七皂苷应用于急性肺损伤治疗,能够有效降低肺静脉高压及SWW浓度,阻止急性肺水肿发展,为临床应用提供重要依据。
  1材料与方法
  1.1实验材料
  1.1.1动物 健康雄性豚鼠:沈阳医学院动物模型中心Sy1703-S003组120只,体重310~350 g,平均340 g。
  1.1.2构模材料 硫化氢(沈阳景泉特种气体厂,H2S,Q1000~07号)。
  1.1.3实验药品、仪器、设备 见表1。
  1.2 实验方法
  ①动物模型的构建:硫化氢(50 mL/L)密室200 m3,将豚鼠全部投放密室经8 h取出,进行乙醚罩吸麻醉,1.5%戊巴比妥钠注射液静脉加强麻醉。②分组:将120只构型豚鼠随机分成四组,每组30只,置于实验台连接实验设备、仪器。Ⅰ组对照组(T0组):100 mL生理盐水肺静脉连续滴注;Ⅱ组(TS组):5 mg地塞米松加生理盐水100 mL肺静脉连续滴注;Ⅲ组(SAT组):生理盐水100 mL加三七皂苷0.25 g配制成浓度0.25%肺静脉连续滴注;Ⅳ组(FS组):在SAT组基础上联合地塞米松5 mg肺静脉连续滴注,测量肺毛细血管静脉压(PCWP)值,记录肺毛细血管静脉压值、血氧饱和度(FIO2)值,各组于造模后7 h、14 h、21 h、24 h观察记录实验数据。
  1.3指标检测及方法

相关热词搜索:豚鼠 硫化氢 治病 损伤 机制

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