基于多元功效成分的山茱萸药材质量标准提升研究

　　[摘要] 建立山茱萸药材中环烯醚萜苷类及三萜酸类化学成分的含量测定方法，为山茱萸药材的质量标准提升提供技术支撑。采用HPLC-UV法，以乙腈-0.1%甲酸水溶液为流动相梯度洗脱，检测波长为240 nm，建立同时测定莫诺苷、马钱苷、当药苷的含量测定方法;采用HPLC-ELSD法，以甲醇-0.5%乙酸铵（87∶13）为流动相，建立同时测定齐墩果酸、熊果酸的含量测定方法。结果显示在上述色谱条件下，莫诺苷、马钱苷、当药苷分别在质量浓度5.335～213.4 mg·L^-1（r = 0.999 9），5.515～220.6 mg·L^-1 （r=1.000 0），1.992～79.68 mg·L^-1（r=1.000 0）线性关系良好，平均加样回收率均在98.49%～99.28%，RSD<2%;齐墩果酸、熊果酸分别在质量浓度7.74～154.8 mg·L^-1（r=0.996 4），10.82～216.4 mg·L-1 （r=0.999 6）线性关系良好，平均加样回收率均在98.11%～99.27%，RSD <3%。表明所建立的方法简单快速，准确可靠，重复性好，可用于客观评价山茱萸药材的品质及其质量控制，为完善山茱萸药材现行质量标准提供了实验数据，对于保证其质量和临床疗效具有重要意义。
　　[关键词] 山茱萸;环烯醚萜苷;三萜酸;药材质量标准制定
　　[收稿日期] 2015-04-12
　　[基金项目] 国家自然科学基金项目（81473538）;教育部霍英东教育基金会高等院校青年教师基金项目（141040）;江苏高校优势学科建设工程资助项目（ysxk-2014）
　　[通信作者] *段金廒，教授，博士研究生导师，Tel： （025）85811116，E-mail： dja@njucm.edu.cn
　　[作者简介] 郭盛，博士，主要从事中药资源化学研究及教学工作，Tel：（025）85811916，E-mail：guosheng@njucm.edu.cn
　　Establishment of quality standard for Corni Fructus based on
　　the multiple bioactive constituents
　　GUO Sheng，  DUAN Jin-ao*，  ZHU Shao-qing，  QIAN Da-wei
　　（Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization， and National and
　　Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative
　　Medicine， Nanjing University of Chinese Medicine， Nanjing 210023， China）
　　[Abstract] This study is to establish the methods for determination of iridoid glycosides and triterpenic acids in Corni Fructus and provide technical support for the quality control of Corni Fructus.Morroniside， loganin and sweroside were determined by HPLC-UV method with acetonitrile and 0.1% formic acid as the mobile phase， and the detective wavelength was set at 240 nm. Oleanolic acid and ursolic acid were determined by HPLC-ELSD method with methanol-0.5% ammonium acetate （87∶13） as the mobile phase. The results showed that  the linear ranges of morroniside， loganin and sweroside were 5.335-213.4 mg·L^-1 （r=0.999 9）， 5.515-220.6 mg·L^-1（r=1.000 0）， 1.992-79.68 mg·L^-1（r=1.000 0）， respectively. The average recoveries of the above three iridoid glycosides were 98.49%-99.28% with RSDs of recoveries being less than 2%.The linear ranges of oleanolic acid and ursolic acid were 7.74-154.8 mg·L-1 （r=0.996 4），10.82-216.4 mg·L-1 （r=0.999 6）， respectively. The average recoveries of the above two triterpenic acids were 98.11%-99.27% with RSDs of recoveries being less than 3%. The method established in this research is simple， rapid and reliable， and can be used for quality control of Corni Fructus. Furthermore， the research provided experimental data for the improvement of present quality standard of Corni Fructus， which has important significance to guarantee its quality and clinical curative effect.

相关热词搜索：山茱萸 药材 质量标准 功效 成分