黄柏类药材颜色数量分类探讨

发布时间:2019-08-29 来源: 幽默笑话 点击:

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  [摘要]通过收集市场上黄柏类药材的商品28批样品,利用分光测色仪测量其外表面、内表面、断面的颜色Lab色彩空间值,采用Ward联接法和平方Euclidean距离对28组数值进行聚类分析。同时邀请“国医大师”金世元教授依据传统经验对28批黄柏样品进行鉴定、质量评价和分级。将传统经验结果与分光测色仪测量分析结果进行比较。结果显示,黄柏类药材可以通过颜色数值聚类成关黄柏和川黄柏,并按照质量进行分类,且与传统经验鉴别结果具有高度一致性。
  [关键词]黄柏类药材;颜色;传统经验鉴别;Lab色彩空间;聚类分析
  [Abstract]Through the investigation of Phellodendron Cortex on the market, and 28 batches of samples were collected. By using spectrophotometer the color values of outer surface, inner surface and cross-section of these samples were measured. These measured color data was translated into 3D structure diagram by using the Lab color space tool. The level difference value, the mean value and the threshold value were calculated based the measured color data of these different batches of samples. All 28 groups measured data was analyzed using the methods of Ward linkage and average Euclidean distance. At the same time, we invited Professor Jin Shiyuan, the ″Chinese medicine master″, to identify, qualityevaluate and grade these 28 batches of Phellodendron Cortex samples base on the traditional experience, then compared the traditional empirical results with the spectrophotometer measurement results. The result showed that, the Phellodendron Cortex could be divided into Phellodendri Amurensis Cortex and Phellodendri Chinensis Cortex by color numerical clustering, and classified according to quality. The classification result has a high degree of consistency with the traditional experience.
  [Key words]Phellodendron Cortex; color; traditional identification; Lab color space; cluster analysis
  黄柏是一种大宗常用中药材,来源于芸香科植物黄皮树Phellodendron chinense Schneid 或黄檗P amurense Rupr 的干燥树皮,前者习称“川黄柏”,后者习称“关黄柏”。 《本草经集注》云:“今出邵陵者,轻薄色深为胜,出东山者,厚而色浅”。《蜀本草图经》云:“黄檗树……皮黄……今所在有,本出房,商,合等小川山谷,皮紧,厚二三分,鲜黄者上,二月、五月采皮,日干。” 宋代《本草图经》说:“今处处有之,以蜀中者佳。”[1]以上诸家所说,与今用之川黄柏相符,且川黄柏质量以“色深、鲜黄”为上。
  颜色是传统鉴别和评价中药材及饮片的最为常用的指标。药材或饮片的质量优劣、变质与否及炮制变化指标均体现在药材的颜色变化。经调查,亳州市场黄柏的商品规格已发生变化,对其进行重新整理并开展优劣鉴别方法研究,对有计划、有组织地利用资源,尽快提升药材质量,满足用药需求是十分必要的。本文拟对市售黄柏类药材,利用分光测色仪对黄柏外表面、内表面和断面的Lab色彩空间数值测量,并结合传统经验鉴别,对黄柏进行品种、等级等外观质量评价。
  1材料与方法
  1.1药材黄柏类药材28份,购买于安徽亳州药材市场,均已切成丝状,其中关黄柏10份,川黄柏18份(4份去栓皮,14份未去栓皮),经“国医大师”金世元教授依照传统经验进行鉴定和优劣评价,见表1。
  1.2仪器采用日本柯尼卡美能达公司的分光测色仪(型号CM700d),参数根据《中国药典》液体颜色检查法第三法 “色差计法”设为:CIE 1976,光源设定为D65,光源视角为10°,镜面光为SCI,UV为100%,量测方式为反射,孔径为3 mm目标罩(含稳定片,型号CMA179),孔径3 mm目标罩(不含稳定片,型号CMA181)。测得数据用Lab色彩空间表示,Lab色彩空间是由1个明度因数L和2个色度因数a,b组成的,L从0到100,a从红色变化到绿色,b从黄色变化到蓝色,它们的值在-120~120 [2] 。
  1.3颜色数值测量每份样品随机取9个黄柏丝个体,分别测量每个个体外表面、内表面和断面的颜色数值,每个表面随机选取1块区域置于分光测色仪下进行测量。

相关热词搜索:黄柏 探讨 数量 颜色 类药

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